Sigma-Aldrich Anti-Adrp/Perilipin-2 Clone Pad-1

Perilipin-2 (UniProt: Q99541; also known as Adipophilin, Adipose differentiation-related protein (ADRP)) is encoded by the PLIN2 (also known as ADFP) gene (Gene ID: 123) in human. ADRP/Perilipin-2 is an intracellular neutral lipid droplet (LD) surface protein that belongs to the PAT family of proteins. The N-terminal region contains a highly conserved hydrophobic PAT domain (aa 1-115) that is involved in lipid droplet stabilization, lipid accumulation, and proteasomal degradation, while the conserved C-terminal domain facilitates its binding to lipid droplets. LDs play an active role in lipid homeostasis, lipid synthesis, metabolism, and transportation. ADRP/Perilipin-2 is a gatekeeper protein that is involved in regulating triacylglycerol storage in adipocyte through the suppression of basal lipolysis apparently through protecting triacylglycerol against hydrolysis and it regulates the recruitment of various proteins involved in lipid synthesis and degradation. Its expression profile is notably high in adipose tissue and liver, with varying levels observed in other tissues. ADRP/Perilipin-2 interacts with other lipid metabolism-related proteins, such as hormone-sensitive lipase. Dysregulation of ADRP/Perilipin-2 expression has been implicated in several metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease. Moreover, ADRP/Perilipin-2 have been associated with certain cancers, suggesting its involvement in tumorigenesis by modulating lipid metabolism within the tumor microenvironment. (Ref: Ye, H., et Al. (1998). Biochem J. 330, 803-9; Zhang, S., et Al. (2016). Sci Rep. 8:6:29539; Conte, M., et Al., Trends Endocrinol & Metab., 27(12), 893-903)

His-tagged, full length, recombinant human ADRP/Perilipin-2.

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected ADRP/Perilipin-2 in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected ADRP/Perilipin-2 in Western Blotting application (Ye, H., et Al. (1998). Biochem J. 330, 803-9; Bartz, R., et Al. (2007). J Proteome Res. 6(8):3256-65; Zhang, S., et Al. (2016). Sci Rep. 8:6:29539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone PAD-1 is a mouse monoclonal antibody that detects ADRP/Perilipin-2.

Purified mouse monoclonal antibody IgG2b in PBS without azide.

2.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.