Sigma-Aldrich Anti-Stabilin-2 Clone 3.1 25 Mig

Fc-tagged recombinant fragment corresponding to 730 amino acids from the N-terminal, extracellular domain of mouse Stabilin-2.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human liver tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Stabilin-2 in human liver tissue sections.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Stabilin-2 in Flow Cytometry application (Klein, D., et Al. (2008). Hepatology. 47(3):1018-31).

Immunohistochemistry Analysis: A representative lot detected Stabilin-2 in Immunohistochemistry application (Martens, J-H., et Al. (2006). J Pathol. 208(4):574-89; Manta, C-P., et Al. (2022). Circulation. 146(23):1783-1799).

Immunofluorescence Analysis: A representative lot detected Stabilin-2 in Immunofluorescence application (Martens, J-H., et Al. (2006). J Pathol. 208(4):574-89; Schledzewski, K., et Al. (2011). 121(2):703-14; Swystun, L.L., et Al. (2018). J Clin Invest. 128(9):4057-4073; Heil, J., et Al. (2021). Nat Commun. 12(1):6963; Manta, C-P., et Al. (2022). Circulation. 146(23):1783-1799).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3.1 is a mouse monoclonal antibody that specifically detects Stabilin-2. It targets an epitope within first 730 amino acids from the extracellular domain of Stabilin-2.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Stabilin-2 (UniProt: Q8R4U0; also known as Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 2, FEEL-2, FAS1 EGF-like and X-link domain-containing adhesion molecule 2, Hyaluronan receptor for endocytosis) is encoded by the Stab2 (also known as Feel2, Hare) gene (Gene ID: 192188) in murine species Stabilin-2 functions as a mammalian endocytic clearance receptor for hyaluronic acid (HA), heparin, advanced glycation end-products, acetylated and oxidized low-density lipoproteins and collagen N-terminal propeptides. Stabilin-2 is a single-pass type I membrane protein and is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce the mature protein that contains a large extracellular domain (aa 29-2464), a transmembrane domain (aa 2465-2485), and a cytoplasmic domain (aa 2486-2559). The extracellular domain contains multiple domains, including EGF-like, fasciclin, and Laminin EGF-like, which are essential for its ligand-binding capabilities and interaction with the endocytic machinery, particularly clathrin and AP-2 complexes. It is highly expressed in sinusoidal endothelial cells of liver, spleen, bone marrow, lymph nodes, in specialised structures of eye, heart, brain and kidney. Stab2 facilitates binding of lymphocyte to sinusoidal endothelium by interacting with Mβ2 and 5β5 integrins. In mice, Stab-2 might assist in separating hyaluronan from the lymph or the blood circulation through the network of endothelial sinuses. The protein also exhibits endocytic scavenger receptor activity. It is suggested that Stabilin-2 may contribute to atherosclerosis and other inflammatory conditions. Additionally, genetic variants in the STAB2 gene have been associated with altered plasma levels of von Willebrand factor, linking this receptor to various hematological disorders. This clone 3.1, mouse monoclonal antibody recognizes both human and mouse full-length stabilin-2, and has been validated by Immunohistochemistry, Immunofluorescence and flow cytometry. (Ref.: Martens, J-H., et Al. (2006). J Pathol. 208(4):574-89; Schledzewski, K., et Al. (2011). 121(2):703-14; Swystun, L.L., et Al. (2018). J Clin Invest. 128(9):4057-4073; Heil, J., et Al. (2021). Nat Commun. 12(1):6963).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.