Sigma-Aldrich Anti-Bcl-2 Clone 100 25 Mig

Apoptosis regulator Bcl-2 (UniProt: P10415; also known as Bcl2) is encoded by the BCL2 gene (Gene ID: 596) in human. Bcl-2 (B-cell lymphoma 2) is a pivotal anti-apoptotic protein belonging to the Bcl-2 family, which regulates cell survival and programmed cell death. Bcl-2 contains four Bcl-2 homology domains (BH1-BH4) and a transmembrane domain that anchors it to intracellular membranes, primarily the outer mitochondrial membrane. Its BH3 domain is critical for interactions with pro-apoptotic proteins such as Bax and Bak, inhibiting their activity and preventing mitochondrial outer membrane permeabilization and cytochrome c release. Bcl-2 is ubiquitously expressed across various tissues, with high levels in immune cells, epithelial cells, and neurons. Bcl-2 interacts with numerous proteins, including BH3-only proteins such as Bad and Bid, as well as non-Bcl-2 family proteins like Beclin-1, modulating autophagy and apoptosis pathways. Dysregulation of Bcl-2 expression is implicated in multiple diseases, including cancer, where its overexpression confers resistance to apoptosis and promotes tumor survival. Bcl-2 is also involved in neurodegenerative diseases like Alzheimer′s, where its imbalance contributes to neuronal death. Therapeutically, targeting Bcl-2 with BH3 mimetics has shown promise in treating hematological malignancies by restoring apoptotic sensitivity in cancer cells.

Thyroglobulin conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Bcl-2.

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:500 Dilution from a representative lot detected Bcl-2 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Bcl-2 in Western Blotting application (Pezzella, F., et Al. (1990). Am J Pathol. 137(2):225-32; Zhou, J., et Al. (2019). Mol Cancer. 18(1):159).

Flow Cytometry Analysis: A representative lot detected Bcl-2 in Flow Cytometry application (Ludwig, L.M., et Al. (2019). Methods Mol Biol. 1877:77-91).

Immunohistochemistry Applications: A representative lot detected Bcl-2 in Immunohistochemistry applications (Pezzella, F., et Al. (1990). Am J Pathol. 137(2):225-32; Sciandra, M., et Al. (2020). J Cell Commun Signal. 14(3):335-347).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 100 is a mouse recombinant monoclonal antibody that detects Bcl-2. It targets an epitope within 14 amino acids from the N-terminal half.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.