Sigma-Aldrich Anti-Cd151 Clone 14A2.H1 25 Mig

CD151 antigen (UniProt: P48509; also known as GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3 (PETA-3), Tetraspanin-24 (Tspan-24)) is encoded by the CD151 (also known as TSPAN24) gene (Gene ID: 977) in human. CD151 is a transmembrane glycoprotein belonging to the tetraspanin family, characterized by four hydrophobic transmembrane domains and two extracellular loops that mediate protein-protein interactions. CD151 acts as a scaffold protein, facilitating the organization of integrin receptors, particularly laminin-binding integrins such as α3β1, α6β1 and α6β4, into specialized tetraspanin-enriched microdomains on the plasma membrane. CD151 plays a pivotal role in cell adhesion, migration, and signaling by modulating integrin-mediated interactions with the extracellular matrix. It is highly expressed in epithelial cells and endothelial cells, with elevated levels observed in various malignancies. CD151 is predominantly localized at intercellular junctions in normal tissues, whereas its cytosolic localization in cancer cells correlates with metastatic potential. CD151 interacts with growth factor receptors such as EGFR and HGFR, influencing pathways like PI3K/Akt and NF- B, which are critical for tumor progression and metastasis. Dysregulation of CD151 contributes to enhanced tumor invasion, angiogenesis, and metastasis in cancers such as lung, prostate, and hepatocellular carcinoma. (Ref.: Zhu, J., et Al., (2021), J Exp Clin Cancer Res, 40(1), 192; Zevian, S.C., et Al., (2015), Cancer Biol Ther, 16(11), 1095-1106, Fitter, S., et Al. (1995), Blood 86(4), 1348-55; Geary, S et Al. (2001) Tissue Antigens, 58, 141 153)

Peripheral blood blast cells from a patient with acute myeloblastic leukemia.

Quality Control Testing

Evaluated by Flow Cytometry in HL-60 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD151 in one million HL-60 cells.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CD151 in Immunohistochemistry applications (Ashman, L.K., et Al. (1991). Br J Haematol. 79(2):263-70).

Flow Cytometry Analysis: A representative lot detected CD151 in Flow Cytometry applications (Ashman, L.K., et Al. (1991). Br J Haematol. 79(2):263-70).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 14A2.H1 is a mouse recombinant monoclonal antibody that detects CD151. It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.