Sigma-Aldrich Anti-Mhc Class Ii I-Ab Clone Af6-120.1

H-2 class II histocompatibility antigen, A beta chain (UniProt: P14483) is encoded by the H2-Ab1 (also known as H2-iabeta) gene (Gene ID: 14961) in murine species. The mouse H-2 class II histocompatibility antigen, specifically the A beta chain (H2-Ab1), is a crucial component of the adaptive immune response, primarily involved in the presentation of peptide antigens to CD4+ T cells. The H2-Ab1 gene is located on chromosome 17 and exhibits a complex expression profile, being predominantly expressed in antigen-presenting cells such as dendritic cells, macrophages, and B lymphocytes. H2-Ab1 protein is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 28-226), a transmembrane domain (aa 227-247), and a cytoplasmic domain (aa 248-265). Dysregulation or mutations in this antigen can lead to autoimmune diseases, as seen in models of diabetes and other inflammatory conditions. The AF6-120.1 antibody has been validated by Flow Cytometry and Immunohistochemistry. It cross-reacts with H-2k and H-2u haplotypes but, does not cross-react with other haplotypes (d, f, q, r, s). (Ref.: Lorber, M.I., et Al. (1982). J Immunol. 128(6):2798-803; Wang, Z., et Al. (2004). J Immunol. 172(10):5924-30).

C57BL/10J mouse splenocytes

Quality Control Testing

Evaluated by Flow Cytometry Analysis with C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg from a representative lot detected MHC class II I-Ab in one million C57BL/6 mouse splenocytes.

Tested Applications

Immunohistochemistry Application: A representative lot detected MHC class II I-Ab in Immunohistochemistry applications (Wang, Z., et Al. (2004). J Immunol. 172(10):5924-30).

Flow Cytometry Analysis: A representative lot detected MHC class II I-A beta chain in Flow Cytometry application (Lorber, M.I., et Al. (1982). J Immunol. 128(6):2798-803).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone AF6-120.1 is a mouse monoclonal antibody that specifically detects MHC class II I-A beta chain. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes but, does not cross-react with other haplotypes (d, f, q, r, s).

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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