Sigma-Aldrich Anti-Epstein Barr Virus Clone 72A1

Epstein Barr Virus particles.

Quality Control Testing

Evaluated by ELISA with His-tagged recombinant Envelope glycoprotein GP350 fragment of Epstein Barr Virus.

ELISA: A representative lot of this antibody detected His-tagged recombinant Envelope glycoprotein GP350 fragment of Epstein Barr Virus in ELISA application.

Tested Applications

Neutralizing Assay: A representative lot of this antibody neutralized the activity of Epstein-Barr Virus (Tanner, J., et Al. (2015). J. Virology. 89(9); 4932-4941; Hoffman, g., et Al., (1980), Proc. Natl. Acad. Sci. USA. 77(5), 2979-2983)

Immunofluorescence Analysis: A representative lot detected Epstein Barr Virus in Immunofluorescence applications (Hoffman, g., et Al., (1980), Proc. Natl. Acad. Sci. USA. 77(5), 2979-2983).

Immunocytochemistry Analysis: A representative lot detected Epstein Barr Virus in Immunocytochemistry application (Gilardini, M., et Al., (2019)., Autophagy.15(4), 652-667).

Flow Cytometry Analysis: A representative lot detected Epstein Barr Virus in Flow Cytometry application (Lopez-Montanes M., et Al., (2017). J. Immunol. 199, 656-665).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 72A1 is a mouse monoclonal antibody that detects Epstein Barr Virus.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Envelope glycoprotein GP350 (UniProt: P03200; also known as Membrane antigen (MA)) is encoded by the BLLF1 open reading frame (Gene ID: 3783713) in Epstein-Barr Virus. Epstein-Barr Virus (EBV) is a ubiquitous gamma-herpesvirus that establishes a lifelong latent infection in human B lymphocytes, with significant implications for oncogenesis and autoimmune diseases. Recent studies have elucidated the molecular mechanisms by which EBV contributes to the pathogenesis of various cancers, including nasopharyngeal carcinoma and gastric cancer. The virus encodes latent membrane protein 1 (LMP1), which mimics a constitutively active receptor, thereby activating NF-kB and JAK/STAT signaling pathways that promote cell proliferation and survival. Moreover, EBV′s role in multiple sclerosis has been reinforced by longitudinal analyses showing a high prevalence of EBV seropositivity in affected individuals. The virus′s ability to modulate host immune responses is further evidenced by its interaction with cellular proteins like TRAF1, linking viral infection to B-cell transformation. EBV′s reactivation is also associated with increased morbidity in patients with severe illnesses such as COVID-19, where it exacerbates immune dysregulation. Ref.: Bernal, K.D.E., et Al., (2023), Virus Res. 334, 199157; Abusalah, M.A.H., et Al., (2020). Pathogens. 9(3), 226; Müller-Durovic, B., et Al., (2025). Trends Immunol. 46(1), 7-16)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.