Sigma-Aldrich Hpc-7 Ms Hematopoietic Precursor Cell

Hematopoietic stem cells (HSCs) are a type of stem cell that differentiate into all other mature blood cells derived from two lineages, myeloid and lymphoid. Hematopoiesis, a process in which HSCs differentiate into other mature blood cell lineages, occurs in the bone marrow, and gives HSCs unique regenerative properties in which they are capable of generating a variety of cell types as well as generating more stem cells in the process. HSCs migrate to injury sites and are useful for tissue repair. HSCs also appear to have potential for therapeutic treatment in inflammatory bowel disease models. HSCs have already displayed some therapeutic benefit experimentally in models used for colitis and Crohn′s disease. Clinical use of HSCs through systemic injection has been quite limited by their rarity and poor tissue retention. Understanding HSC recruitment remains an important topic for improving stem cell-based therapies. HPC-7, a mouse HSC cell line, was developed to further understand hematopoiesis and functionality of HSCs. The HPC-7 cell line retains many cell characteristics typical of fetal-derived hematopoietic precursors such as being c-kit+, CD34+, CD44+, Sca-1-, and Thy1.2-. Transcription factors expressed by the HPC-7 cell line are also consistent with characterized HSCs. HPC-7 cells can be differentiated through the addition of different cell factors and have been shown to change cell-surface expression post-differentiation. HPC-7 cells also retain the self-renewal properties characteristic of HSCs. Source: Mouse LH2 cDNA was retrovirally expressed in embryonic stem cells and subjected to differentiation assay in vitro. This led to the generation of multipotent hematopoietic precursor cells labeled HPC-7.ReferencesPinto do O P. 1998. Expression of the LIM-homeobox gene LH2 generates immortalized Steel factor-dependent multipotent hematopoietic precursors. The EMBO Journal. 17(19):5744–5756.

The HPC-7 cell line retains many cell characteristics of fetal-derived hematopoietic precursors such as being c-kit+, CD34+, CD44+, Sca-1-, and Thy1.2-.

The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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