Sigma-Aldrich 3Dgro Hu Liver Organoids (ID Cs235474)

Patient derived organoids (PDO) are tissue derived, novel in vitro 3D cell models that recapitulate some key features of the respective organ, thus representing a more in vivo-like alternative to traditional 2D cell cultures. In addition, PDOs offer possible means to reduce animal model use. PDO maybe derived from resections or biopsy of adult tissue and are phenotypically and genetically stable in culture. PDO are an ideal platform for drug screening, disease modeling and tumor immunity studies, and may also be modified by gene editing.

The 3dGRO^® human liver organoid biobank is a collection of tissue-derived normal non-diseased human liver organoids from numerous patients. The biobank contains liver PDOs that are highly viable and express high levels of albumin, HNF4-a, FOXA2, SOX17, CK7, CK19, E-Cadherin, PCK2 along with important cytochrome P450 (CYP) enzymes activities (Cyp3A4, Cyp2C9, Cyp2B6, Cyp2D6, Cyp1A2).

Cell Line Characteristics

• Sex: Female
• Age: 57 Years
• Organ: Liver
• Disease: Normal
• Medications: Advil, Tylenol, Zosyn
• Patient History: COD: Anoxia, BMI: 30.27, Alcohol: binge drinking 3d/week X 10 yrs; Tobacco: 1 pack every 3-4 days X 3 yrs; Drugs: Methamphetamine 2d/week X 3 yrs. Used Fentanyl

3dGRO^® organoids were derived utilizing HUB Organoid Technology. The purchaser of this product shall agree to HUB′s terms of use (listed below), which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this product to purchaser.

Cryopreserved patient derived human liver organoids (PDOs)

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.