Sigma-Aldrich Anti-Brd2 Clone 1M5 Zoomab Rb Mono

Bromodomain-containing protein 2 (UniProt: P25440; also known as O27.1.1) is encoded by the BRD2 (also known as KIAA9001, RING3) gene (Gene ID: 6046) in human. Bromodomain-containing protein 2 (BRD2) is a member of the bromodomain and extraterminal (BET) protein family, functioning as a pivotal epigenetic regulator by recognizing acetylated lysine residues on histones, thereby modulating chromatin structure and gene expression. BRD2 contains two tandem N-terminal bromodomains that facilitate its binding to acetylated histones, particularly histone H4. The C-terminal conserved extra-terminal domain is crucial for protein-protein interactions. BRD2 is ubiquitously expressed across various tissues. BRD2 is implicated in numerous cellular processes, including cell cycle progression, apoptosis, and immune response modulation, and regulation of alternative splicing of several hundred genes. Its dysregulation contributes to tumorigenesis by influencing oncogene expression and maintaining genomic stability. BRD2 also involved in neurological disorders, inflammatory diseases and BRD2 mutants have been associated with increased susceptibility to juvenile myoclonic epilepsy. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ferri, E., et Al., (2016), Biochem Pharmacol, 106, 1-18; Ji, Y., et Al., (2024), J Pharmacol Exp Ther, 389(3), 277-288; Werner, M.T., et Al., (2020), J Biol Chem, 295(7), 1898-1914).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human BRD2.

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected BRD2 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected BRD2 in lysate from mouse brain tissue and a 1:1,000 dilution from a representative lot detected BRD2 in lysate from rat brain tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected BRD2 in human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 from a representative lot detected BRD2 in HEK-293 cells.

Affinity Binding Assay: A representative lot of this antibody bound with BRD2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1M5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects BRD2. It targets an epitope within 22 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.