Sigma-Aldrich Anti-Glucagon Recep Cl 2B13 Zoomab Rb

Glucagon receptor (UniProt: P47871; also known as GL-R) is encoded by the GCGR gene (Gene ID: 2642) in human. The glucagon receptor (GCGR) is a g protein-coupled receptor (GPCR) that consists of seven transmembrane helices. It has an extracellular N-terminus that binds glucagon, a peptide hormone, and an intracellular C-terminus that interacts with g proteins. The receptor undergoes conformational changes upon glucagon binding, activating intracellular signaling pathways. The glucagon receptor is crucial for glucose homeostasis. When glucagon binds to its receptor, it activates adenylate cyclase, leading to an increase in cyclic AMP (cAMP) levels. This cascade results in the stimulation of gluconeogenesis and glycogenolysis in the liver, raising blood glucose levels. Additionally, GCGR plays a role in lipid metabolism and may influence appetite regulation. GCGR is predominantly expressed in the liver, where it exerts its primary metabolic effects. It is also found in lower levels in other tissues, including the pancreas, kidney, and brain. The expression of the glucagon receptor can be influenced by various factors, including hormonal signals and metabolic states. Increased GCGR activity can lead to hyperglycemia and is implicated in type 2 diabetes mellitus. Conversely, decreased receptor function can contribute to hypoglycemia. Additionally, GCGR has been studied as a potential target for therapeutic interventions in obesity and metabolic syndrome. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain of human Glucagon receptor.

Quality Control Testing

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Glucagon receptor in rat kidney tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Glucagon receptor in lysate from human liver tissue tissue.

Affinity Binding Assay: A representative lot of this antibody bound with Glucagon Receptor peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Glucagon receptor in human and rat liver tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Glucagon receptor in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2B13 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Glucagon receptor. It targets an epitope within 15 amino acids from the extracellular domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.