Sigma-Aldrich Anti-Il2Rb Clone 4D2 Zoomab Rb Mono

Interleukin-2 receptor subunit beta (UniProt: P14784; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75 (p75), CD122) is encoded by the IL2RB (also known as IL15RB) gene (Gene ID: 3560) in human. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. IL2RB is a single-pass type I membrane protein. It is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-265), and a long cytoplasmic domain (aa 266-551). IL2RB contains a WSXWS motif (aa 220-224), which is essential for its proper folding and thereby efficient intracellular transport and cell-surface receptor binding. The protein also has a Box 1 motif (aa 278-286) that is required for JAK interaction and/or activation. This receptor is predominantly expressed in T cells, but it is also found in other immune cells, including B cells and natural killer cells. Dysregulation of IL2RB expression or function has been implicated in several diseases, including autoimmune disorders and cancer. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, Z., et Al. (2019). J. Exp. Med. 216(6);1311 1327); Y, Liao., et Al. (2022). BioMed. Res. Int. 2022:2114699).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, extracellular domain of human IL2RB.

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IL2RB in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IL2RB in lysates from HEK-293T cells and rat spleen tissue.

Affinity Binding Assay: A representative lot of this antibody bound IL2RB peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IL2RB in human spleen tissue and human bone marrow tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 4D2 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects IL2RB. It targets an epitope within 22 amino acids from the N-terminal, extracellular domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.