Sigma-Aldrich Anti-Cd209 Clone 4B5 Zoomab Rb Mono

CD209 antigen (UniProt: Q9NNX6; also known as C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN; DC-SIGN1)) is encoded by the CD209 (also known as CLEC4L) gene (Gene ID: 30835) in human. CD209 is a C-type lectin receptor primarily expressed on dendritic cells and macrophages. CD209 consists of an extracellular domain containing a carbohydrate recognition domain (CRD), a neck region that mediates tetramerization, a transmembrane domain, and a cytoplasmic tail essential for intracellular signaling. CD209 plays a critical role in pathogen recognition and immune modulation by binding high-mannose glycans present on pathogens such as HIV, SARS-CoV-2, and Mycobacterium tuberculosis. This interaction facilitates pathogen uptake through endocytosis and phagocytosis, triggering downstream signaling pathways that regulate innate and adaptive immunity. CD209 is predominantly localized to the plasma membrane and endosomal compartments, where it mediates antigen presentation and cytokine production. It interacts with intracellular proteins such as LSP1 and other signaling molecules to modulate immune responses. Dysregulation of CD209 is implicated in various diseases, including infectious diseases, autoimmune disorders, and cancer, where altered expression or function contributes to immune evasion and chronic inflammation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to amino acids from the C-terminal extracellular domain of human CD209.

Quality Control Testing

Evaluated by Western Blotting in LoVo cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD209 in LoVo cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound with CD209 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD209 in human colon and human liver tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD209 in Human Peripheral Blood Monocytes Derived Mature Dendritic Cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD209/DC-SIGN in THP-1 cells .

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 4B5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD209. It targets an epitope within 16 amino acids from the C-terminal extracellular domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.