Sigma-Aldrich Anti-Cd79A Clone 2C16 Zoomab Rb

B-cell antigen receptor complex-associated protein alpha chain (UniProt: P11912; also known as Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein) is encoded by the CD79A (also known as IGA, MB1) gene (Gene ID: 973) in human. CD79A is a single-pass type I membrane protein that plays a central role in B-cell receptor (BCR) signaling and is essential for B-cell development and function. BCR complex is composed of a membrane-bound immunoglobulin molecule noncovalently linked with CD79A and CD79B heterodimer acting as the signaling components of the BCR complex. CD79A contains an immunoreceptor tyrosine-based activation motif (ITAM) within its cytoplasmic domain, which is phosphorylated upon antigen binding, initiating downstream signaling cascades. This phosphorylation facilitates the recruitment of Src-family kinases, such as spleen tyrosine kinase (Syk), leading to the activation of pathways critical for B-cell survival, proliferation, and differentiation. CD79A has emerged as a key player in pathological conditions, including B-cell malignancies such as diffuse large B-cell lymphoma (DLBCL) and B-cell precursor acute lymphoblastic leukemia (BCP-ALL), where its expression influences tumor proliferation and central nervous system (CNS) infiltration. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tkachenko, A., et Al., (2023), Int J Mol Sci. 25(1), 10).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to the C-terminal extracellular domain of human CD79A.

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD79A in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD79A in Ramos and Daudi cell lysates.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD79A in one million Ramos cells.

Affinity Binding Assay: A representative lot of this antibody bound with CD79A peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD79A in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2C16 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD79A. It targets an epitope within the C-terminal cytoplasmic domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.