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Sigma-Aldrich RK-13 CELL LINE RABBIT KIDNEY
List Price $1,060.00 Your Price $1,060.00
Sigma-Aldrich RK-13 CELL LINE RABBIT KIDNEY - SIALGSK (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALGSK-00021715-1VL
MFG.PART: 00021715-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
RK-13 Cell Line from rabbit, rabbit kidney, 00021715
biological source
rabbit kidney
growth mode
Adherent
karyotype
2n=44, stem-line = 56 (=22%)
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Rabbit kidney
Cell Line Description
RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
Application
Virus studies
Culture Medium
EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.
Other Notes
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| SKU | SIALGSK-00021715-1VL |
|---|---|
| Supplier Part Number | 00021715-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALGSK |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |