Revvity HTRF Cellular GTP / Gi protein Binding Kit, 20,000 assay points

This cellular GTP / Gi binding assay is a fluorescent and homogeneous format to measure Gi protein activity in cells at the level of the GDP/GTP nucleotide exchange that characterizes GPCR activation. As such, it has the advantage of monitoring the activation of GPCRs at the level of one of the earliest receptor-mediated events. It therefore provides insight into the upstream portion of GPCR signaling, useful when studying functional responses.

HTRF assays offer many advantages over other technologies:

• Homogeneous add-and-read format
• No wash steps
• Low background
• Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
• Stable signal, providing flexibility in readout time or size of assays