Revvity pHSense Human IgG4 Negative Control, 50µg

pHSense Eu Fab reagents are designed to monitor antibody or anti-drug conjugate (ADC), as well as receptor- and membrane protein-mediated internalization in real time. To confidently attribute observed effects to specific antigen binding, it is crucial to include proper controls. Test antibodies may engage in non-specific interactions - particularly when their Fc regions bind to Fc receptors on cells - which can contribute to background signal. This is where negative control antibodies become essential in pHSense assays. They help distinguish true signal from background noise by revealing non-specific binding effects, thereby enhancing the reliability of your internalization measurements.

The pHSense Human IgG4 Negative Control is a full-length recombinant IgG4 antibody, composed of both heavy and light chains. It serves as an ideal isotype control when evaluating fully human, humanized, or chimeric antibodies in conjunction with pHSense reagents.