Revvity HTRF Human Total SKP2 Detection Kit, 10,000 assay points

S-phase kinase-associated protein 2 (SKP2), also known as FBXL1, is a prominent member of the family of F-box proteins. It functions as an E3 ligase mainly through the formation of an SCF-SKP2 complex. SKP2 is well known for its capacity to regulate the cell cycle. It can ubiquitinate various cell cycle regulators and induce their hydrolysis, including CKIs p21, p27, and p57, as well as the cell cycle protein E (cyclin E) and the cell cycle regulator c-Myc. As a driver of oncogenesis and drug resistance, SKP2 is an attractive target for small-molecule inhibitors and protein degradation strategies aimed at restoring cell cycle checkpoints.

HTRF assays offer many advantages over other technologies:

• Homogeneous add-and-read format
• No wash steps
• Low background
• Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
• Stable signal, providing flexibility in readout time or size of assays