Revvity HTRF Human and Mouse Phospho-CDC6 (Ser54) Detection Kit, 500 Assay Points

CDC6, or Cell Division Cycle 6, is a member of the AAA+ ATPase family and plays a crucial role in the regulation of DNA replication. It is an essential component of the pre-replication complex, facilitating its assembly at origins of replication to ensure that DNA replication occurs only once per cell cycle. Beyond its role in replication initiation, CDC6 also contributes to the activation and maintenance of cell cycle checkpoints, which are vital for preserving genomic stability.

HTRF assays offer many advantages over other technologies:

• Homogeneous add-and-read format
• No wash steps
• Low background
• Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
• Stable signal, providing flexibility in time of readout or size of assays