Revvity AlphaLISA SureFire Ultra Human and Mouse High Specificity Phospho-SMAD3 (Ser423/425) Detection Kit, 10,000 Assay Points

SMAD3 is an intracellular signal transducer and transcription factor that mediates TGF-β and activin signaling to regulate cell growth, differentiation, and extracellular matrix production. Upon TGF-β receptor activation, SMAD3 is phosphorylated, enabling formation of complexes with SMAD4 that translocate to the nucleus. The SMAD3/SMAD4 complex regulates transcription of genes including collagens, fibronectin, and cyclin-dependent kinase inhibitors. SMAD3 plays context-dependent roles as both a tumor suppressor and tumor promoter. Loss of SMAD3 contributes to colorectal cancer, while excessive SMAD3 activation drives pathological fibrosis in multiple organs.

The AlphaLISA SureFire Ultra Human and Mouse High Specificity Phospho-SMAD3 (Ser423/425) is a sandwich immunoassay for the quantitative detection of phospho-SMAD3 (Ser423/425)  in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies