Revvity AlphaLISA SureFire Ultra Human MyD88 Dimer Detection Kit, 10,000 Assay Points

MYD88 homodimers represent the initial oligomeric state in the assembly of the myddosome signaling complex that transduces signals from TLRs and IL-1Rs. Upon receptor activation, MYD88 molecules undergo conformational changes promoting death domain interactions and dimer formation. MYD88 dimers serve as nucleation sites for higher-order oligomerization that recruit and activate IRAK family kinases. Oncogenic MYD88 L265P mutations enhance MYD88 dimerization and spontaneous myddosome formation, driving chronic NF-κB signaling in B-cell malignancies. Targeting MYD88 dimerization represents a therapeutic strategy for MYD88-mutant lymphomas.

The AlphaLISA SureFire Ultra Human MyD88 Dimer is a sandwich immunoassay for the quantitative detection of MyD88 dimer in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies