Revvity AlphaLISA SureFire Ultra Human and Mouse Total AKT2 Detection Kit, 500 Assay Points

AKT2 is a serine/threonine protein kinase and a key member of the PI3K/AKT signaling pathway, playing critical roles in glucose metabolism, insulin sensitivity, and cellular survival. AKT2 is activated by phosphorylation at Thr308 and Ser473 downstream of PI3K, promoting glucose uptake, glycogen synthesis, and lipogenesis while inhibiting gluconeogenesis. It is particularly important in muscle and adipose tissue for maintaining glucose homeostasis. Mutations or altered expression of AKT2 are associated with type 2 diabetes, metabolic syndrome, and various cancers including breast, ovarian, and pancreatic carcinomas. In oncology, AKT2 promotes tumor cell survival, proliferation, and metastasis while conferring resistance to apoptosis. Therapeutic targeting of AKT2 through selective inhibitors represents a promising strategy for both metabolic disorders and cancer treatment.

The AlphaLISA SureFire Ultra Human and Mouse Total AKT2 is a sandwich immunoassay for the quantitative detection of total AKT2 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies