Revvity AlphaLISA SureFire Ultra Human Total MSH3 Detection Kit, 500 Assay Points

MutS Homolog 3 (MSH3) is a DNA mismatch repair protein that forms the MutSβ complex with MSH2 to recognize and repair insertion-deletion loops (IDLs) and larger mismatches in DNA. MSH3 is particularly important for repairing slipped-strand intermediates that arise during DNA replication in repetitive sequences, including microsatellites. The MutSβ complex initiates repair by recruiting MLH1-PMS2 (MutLα) and other downstream factors to excise and resynthesize the error-containing DNA strand. Deficiency in MSH3 leads to microsatellite instability (MSI) and increased mutation rates, particularly in repetitive sequences. MSH3 mutations are associated with certain hereditary cancer syndromes and contribute to tumorigenesis through genomic instability. In cancer therapy, MSH3 status influences response to DNA-damaging agents and immunotherapy, as MSI tumors often exhibit enhanced immunogenicity.

The AlphaLISA SureFire Ultra Human Total MSH3 is a sandwich immunoassay for the quantitative detection of total MSH3 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies