Revvity AlphaLISA SureFire Ultra Mouse Phospho-STING (Ser365) Detection Kit, 500 Assay Points

Stimulator of Interferon Genes (STING) is an adaptor protein that mediates innate immune responses to cytosolic DNA. Upon binding of cyclic dinucleotides (CDNs) produced by cGAS in response to DNA sensing, STING undergoes conformational changes and translocates from the ER to the Golgi, initiating TBK1- and IRF3-dependent induction of type I interferons and inflammatory cytokines. STING plays critical roles in antiviral defense, cancer immunosurveillance, and autoimmune pathogenesis. STING agonists are under investigation for cancer immunotherapy, aiming to boost antitumor immunity, while inhibitors are being explored for autoinflammatory and autoimmune diseases driven by excessive type I IFN signaling.

The AlphaLISA SureFire Ultra Mouse Phospho-STING (Ser365) is a sandwich immunoassay for the quantitative detection of phospho-STING (Ser365) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies