Revvity AlphaLISA SureFire Ultra Human and Mouse Phospho-FOXO1 (Ser256) Detection Kit, 100 Assay Points

Forkhead Box O1 (FOXO1) is a transcription factor that regulates genes involved in glucose metabolism, cell cycle arrest, apoptosis, and oxidative stress resistance. FOXO1 activity is controlled by AKT-mediated phosphorylation, which promotes nuclear exclusion and proteasomal degradation. Dephosphorylated FOXO1 translocates to the nucleus and activates target genes including glucose-6-phosphatase, PEPCK, and p27Kip1. FOXO1 plays critical roles in hepatic gluconeogenesis and functions as a tumor suppressor in cancer. Dysregulation of FOXO1 is implicated in type 2 diabetes and cancer, making it a therapeutic target for metabolic disorders and malignancies.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-FOXO1 (Ser256) is a sandwich immunoassay for the quantitative detection of phospho-FOXO1 (Ser256) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies