Revvity AlphaLISA SureFire Ultra Human IRAK1 Dimer Detection Kit, 500 Assay Points

IRAK1 homodimers represent an active signaling state that forms during TLR/IL-1R pathway activation and contributes to signal amplification. Following IRAK4-mediated phosphorylation, IRAK1 molecules undergo trans-autophosphorylation and form dimeric complexes with enhanced kinase activity. The dimeric form shows increased catalytic efficiency and enhanced ability to recruit TRAF6, amplifying the inflammatory signal. Hyperactive IRAK1 dimerization contributes to excessive inflammation in autoimmune diseases and supports oncogenic signaling in MYD88-mutant cancers. The dimer interface represents a potential therapeutic target for selective IRAK1 inhibition.

The AlphaLISA SureFire Ultra Human IRAK1 Dimer is a sandwich immunoassay for the quantitative detection of IRAK1 dimer in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies