Revvity AlphaLISA SureFire Biotin-Free Human Phospho-Cyclin E1 (Thr77) Detection Kit, 500 Assay Points

Cyclin E1 is a regulatory protein that controls the G1-to-S phase transition of the cell cycle by activating cyclin-dependent kinase 2 (CDK2). The Cyclin E1-CDK2 complex phosphorylates substrates including retinoblastoma protein and p27Kip1, promoting initiation of DNA replication. Cyclin E1 overexpression or amplification occurs in numerous cancers including breast, ovarian, and endometrial carcinomas, driving uncontrolled proliferation and chromosomal instability. Low molecular weight forms of Cyclin E1 exhibit enhanced oncogenic activity and resistance to CDK inhibitors. Selective CDK2 inhibitors are being developed as therapeutics for Cyclin E1-amplified cancers.

The AlphaLISA SureFire Biotin-Free Human Phospho-Cyclin E1 (Thr77) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin E1 (Thr77) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies