Revvity Alpha Surefire Ultra Multiplex Phospho/Total Pdgf Receptor Assay Kit, 100 Assay Points

The Alpha SureFire Ultra Multiplexp-PDGF Receptor β (Tyr751) + Total PDGF Receptor β assay kit is used to measure both phosphorylated and total levels of endogenous PDGF Receptor β in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated PDGF Receptor β analysis, and the 545 nm (Tb) signal corresponds to the total PDGF Receptor β analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

• No-wash steps, no separation steps
• Multiplexing assay
• Sensitive detection
• No interference from exogenous antibodies
• Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.