Revvity Alphalisa Surefire Ultra Human Phospho-C-Fos (Ser32) Detection Kit, 500 Assay Points

Protein c-Fos is encoded by the FOS gene, a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. The Activator Protein-1 (AP-1) complex is formed when c-Fos and c-Jun form a heterodimer. This complex binds DNA at specific AP-1 sites in the promoter and enhancer regions of target genes, converting extracellular signals into changes in gene expression. Additionally, posttranslational alterations brought on by phosphorylation by various kinases, such as MAPK, CDC2, PKA, or PKC, regulate its activity. C-Fos plays an important role in numerous cellular functions, including cell proliferation, differentiation, survival, genes associated with hypoxia, and has been found to be overexpressed in a variety of cancers.

The AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-c-FOS (Ser32) in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening