Revvity AlphaScreen IgG (Protein A) Detection Kit, 100 Assay Points

This bead kit can be used for many applications, including:

• Antibody detection
• Antibody binding
• Binding assays
• Enzymatic assays

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. This kit contains enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.