Revvity AlphaLISA. SureFire Ultra. Human Phospho-Tau Thr217 Detection Kit, 500 Assay Points

Tau proteins, derived from tubulin-associated units, play a pivotal role in the pathogenesis of Alzheimer’s disease (AD). Hyperphosphorylated Tau aggregates form filaments, which can further condense into neurofibrillary tangles. These Tau aggregates, often referred to as ‘seeds,’ can propagate pathology from cell to cell, similar to prion transmission. Effective therapeutic strategies for AD include medications that modulate Tau hyperphosphorylation and reduce Tau aggregation.

The AlphaLISA SureFire Ultra Human Phospho-Tau (Thr217) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Tau in cellular lysates, using Alpha technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput drug screening