Revvity AlphaLISA Human Neurofilament L (NF-L) Detection Kit, 5,000 Assay Points

Neurofilament Light chain protein, also known as Neurofilament Light polypeptide, NF-L, NFL or NEFL is a member of intermediate filament protein family, selectively expressed in neurons. NF-L (68 kDa) associated with neurofilament-heavy chain (NF-H, 200 kDa), medium chain (NF-M, 125 kDa) and alpha-internexin, forms the structure of axons. Depending on conditions, different levels of NF-L are released during brain development, maturation, aging and in numerous neurodegenerative diseases proportionally to the degree of axonal damage. Many studies have demonstrated that NF-L can be used as a biomarker for disease monitoring and more broadly as a neuronal damage marker but also to check the differentiation into neurons in IPSc models for example.

Formats

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.