Revvity AlphaLISA. SureFire Ultra. Human & Mouse Total TRAF3 Detection Kit, 500 Assay Points

TNF receptor-associated factor 3 (TRAF3) is an adaptor protein involved in the regulation of immune responses and apoptosis. TRAF3 is a key mediator in the signaling pathways of various receptors, including TNF receptors, Toll-like receptors, and B-cell receptors. It activates the IKK complex, leading to the activation of NF-κB, which then translocates to the nucleus to promote the expression of target genes. TRAF3 plays a significant role in regulating both innate and adaptive immunity. Dysregulation of TRAF3 can lead to autoimmune diseases and chronic inflammatory conditions. Mutations or deletions of TRAF3 can contribute to the development of cancers, including lymphomas and multiple myeloma.

The AlphaLISA SureFire Ultra Human and Mouse Total TRAF3 Detection Kit is a sandwich immunoassay for the quantitative detection of total TRAF3 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening