Revvity AlphaLISA. SureFire Ultra. Human & Mouse Cleaved PARP1 D214 Detection Kit, 500 Assay Points

Cleaved Poly (ADP-ribose) polymerase 1 (PARP1) is a marker of apoptosis, a form of programmed cell death. PARP1 is a DNA repair enzyme that becomes activated in response to DNA damage. During apoptosis, PARP1 is cleaved by caspases, specifically at Asp214, rendering it inactive and preventing DNA repair, which facilitates the apoptotic process. Cleaved PARP1 serves as an important biomarker for apoptosis in cancer research and therapeutic assessment.

The AlphaLISA SureFire Ultra Human and Mouse Cleaved PARP1 D214 Detection Kit is a sandwich immunoassay for the quantitative detection of Cleaved PARP1 D214 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening