Revvity AlphaLISA. SureFire Ultra. Human & Mouse Phospho-Raf-1 Ser259 Detection Kit, 100 Assay Points

RAF proto-oncogene serine/threonine-protein kinase has numerous aliases, including Raf-1, c-Raf, and proto-oncogene c-RAF. Raf-1 is an enzyme belonging to the subfamily of membrane associated GTPases. Raf-1 is a principal component of the mitogen-activated protein kinase (MAPK) pathway, specifically the ERK1/2 signaling cascade 1. Raf-1 regulates cell growth and division, playing a key role in cell proliferation.  It is also involved in cell survival pathways and metabolic processes within cells. This protein is associated with various cancers, including bladder urothelial carcinoma, skin cutaneous melanoma, and uterine corpus endometrial carcinoma.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-Raf-1 (Ser259) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Raf-1 in cellular lysates, using Alpha technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput drug screening