Revvity The AlphaLISA SureFire Ultra Phospho-RSK1 (Tyr573) assay

Serine/threonine-protein kinase acts downstream from ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81, and NR4A1/NUR77. It regulates translation through RPS6 and EIF4B phosphorylation and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing the pro-apoptotic function of BAD and DAPK1.

Upon extracellular signal or mitogen stimulation, RSK1 is phosphorylated at Thr-573 in the C-terminal kinase domain (CTD) by MAPK1/ERK2 and MAPK3/ERK1.

The AlphaLISA™ SureFire® Ultra™ Phospho-RSK1 (Tyr573) assay is a sandwich immunoassay for quantitative detection of phospho-RSK1 in cellular lysates using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening