Revvity Immunoassay buffer, 10X, 10 mL

Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. AlphaLISA Immunoassay Buffer (IAB) is a broadly-applicable buffer, used in most AlphaLISA immunoassays. This buffer ontains detergent and low salt, SO it can be used to lyse cells in some assays. It is generally sufficient to expose the protein epitopes of membrane proteins for Alpha assay detection.

10X formulation: 250 mm HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100 and 0.5% Proclin-300