Revvity AlphaLISA SureFire Ultra Human Total RAPTOR Detection Kit, 50,000 Assay Points

Regulatory-associated protein of mTOR, also known as raptor, RPTOR, or KIAA1303, is an adapter protein encoded by the RPTOR gene. RAPTOR is part of the mTOR complex1 (mTORC1) which also includes mTOR, GL, and DEPTOR. mTORC1 is a growth regulator that detects and combines a variety of dietary and environmental stimuli, including growth factors, energy levels, cellular stress, and amino acids. mTOR inhibitors (PIKKs) include rapamycin and its analogs, which have demonstrated tumor responses in human studies. mTORC1 is associated with breast, ovarian, cervical, liver, gastric, colorectal, lung, and brain cancers.

The AlphaLISA SureFire Human Total RAPTOR Detection Kit is a sandwich immunoassay for the quantitative detection of total RAPTOR in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening