Revvity Alphalisa Surefire Ultra Human Phospho-Cyclin B1 (Ser126) Detection Kit, 10,000 Assay Points

G2/mitotic-specific cyclin-B1 (Cyclin B1) is a key component in the control of cell cycle progression from G2 to M phase and has been implicated in the tumorigenesis and the development of malignancy. Under DNA damage conditions, Cyclin B1 becomes upregulated, presumably already in the DNA replication phase (S-phase). This upregulation is controlled by SOG1, which in turn is activated by the checkpoint kinases ATM and ATR, which sense DNA damage. Cyclin B1/CDK1 regulate mitochondrial bioenergetics for cell cycle progression and tumor response to therapy. Cyclin B1 is associated with breast, colorectal, ovarian, lung, and pancreatic cancer.

The AlphaLISA SureFire Human Phospho-Cyclin B1 (Ser126) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin B1 (Ser126) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening