Revvity AlphaLISA Biotin-Free Human IL-15 Detection Kit, 5,000 Assay Points

The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

Features:

• No-wash steps, no separation steps
• Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Results in less than 3 hours

Formats:

• Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Human Interleukin 15 (IL15) is a cytokine produced as a precursor maturing into a 114 amino acid glycoprotein. IL15 is generated by multiple tissues (placenta, skeletal muscle, kidney, lung, heart, and monocytes/macrophages). IL15 acts mainly on T and B cells and it is well known as a key cytokine in natural killer cell development, proliferation, and survival. IL15 binds with high affinity to the IL15 receptor a and forms a complex with the a and ? chains of the IL2 receptor for signal transduction. It can regulate inflammatory response and NK cell activation during immune responses. IL15 signalling has become a focus of interest for therapeutic intervention especially in autoimmune diseases like inflammatory bowel disease, systemis lupus erythematosus, inflammatory synivitis, psoriasis, diabetes mellitus, asthma bronchiale, rheumatoid arthritis, and T-cell leukemia.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.