Revvity AlphaLISA Human CCL3/MIP-1α Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

C-C Motif Chemokine 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1a), contains 70 amino acids in its mature form. It is a factor produced by macrophages that causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. CCL3 is involved in the acute inflammatory state and in the recruitment and activation of polymorphonuclear leukocytes. It binds to CCR1, CCR4, and CCR5. CCL3 is known mainly for its activity as a chemotactic cytokine, but may also have a potential role in host defense as a direct antiviral agent. CCL3 has been reported to show a significant direct antiviral activity against HSV-1. It is one of the major HIV-suppressive factors produced by CD8+ T-cells. CCL3 is also an inhibitor of haematopoietic stem cell proliferation.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.