Revvity HTRF Mouse Total STING Detection Kit, 10,000 Assay Points

The Mouse Total STING cellular assay monitors mouse total STING, and can be used as a normalization assay with the Mouse Phospho STING kit. This kit is compatible with the buffers from the Mouse Phospho STING kit, SO the same lysate can be used for analyses of both the phosphorylated and the total protein populations.

Following pathogen infection and binding of dsDNA to the cytoplasmic sensor cGAS, STING protein is phosphorylated by TBK1. This enables, its binding to IRF3 which induces IFNs type 1 production and other immune responses. The STING pathway is then switched off by STING degradation involving autophagy.