Revvity HTRF Human Phospho-Phospholamban (Thr17) Detection Kit, 10,000 Assay Points

This cell-based assay enables the detection of Phosphorylated Phospholamban (PLN) at Threonine 17. The phospho assay along with the total PLN assay are ideal for monitoring the activation of CaMKII. The increase in intracellular Ca2+ causes the activation of CaMKII, which in turn phosphorylates PLN at the Thr17 residue. Dephosphorylated PLN inhibits SERCA2a, whereas phosphorylation of PLN at either the Ser16 site by PKA or the Thr17 site by CaMKII reverses this SERCA2a inhibition. The PLN phosphorylation state regulates the activity of this Ca2+ pump.