Revvity HTRF Human Phospho-MLKL (Ser358) Detection Kit, 10,000 Assay Points

This HTRF cell-based assay conveniently and accurately detects phosphorylated MLKL at Ser358.

MLKL (Mixed lineage kinase domain-like protein) is a key mediator of apoptotic and necrotic cell death, as well as of inflammatory pathways downstream from TNFR1 and other receptors. MLKL is phosphorylated at Serine 358 by RIPK3, and is considered to be a biomarker of the initiation of RIPK1/RIPK3-dependent necroptosis.

Necroptosis is a programmed necrotic cell death pathway, also called "inflammatory cell death", which is closely associated with pathologies including inflammatory and neurodegenerative diseases, as well as cancer. MLKL has therefore become an important drug target for the pharmaceutical industry. Therapeutic strategies consist in developing specific small-molecule kinase inhibitors, such as the well-known Necrostatin-1 and Necrostatin-1s.