Revvity HTRF Human Phospho-IκKα (Ser176/180) Detection Kit, 10,000 Assay Points

Inhibitor of nuclear factor kappa-B kinase subunit alpha (IKKα) also known as IKK1 is a protein kinase encoded by the CHUK human gene. IKKα is part of the IκB kinase complex (IKK) composed of the catalytic subunits IKKα and IKKβ and the regulatory subunit NF-κB essential modulator (NEMO, IKKγ).

The activation of the IKK complex depends on the phosphorylation of IKKβ (Ser177/180) or IKKα (Ser176/Ser 180) resulting in complex conformational change and kinase activation. IKKα is a key regulator of the NF-κB pathway downstream of several proinflammatory factors including interleukin-1, tumor necrosis factor alpha (TNFα) and toll-like receptor agonists. Although IKKα and IKKβ are highly homologous sharing over 50% of structural similarity they have different downstream substrates and non-overlapping functions.