Revvity HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 500 Assay Points

This HTRF cell-based assay enables the rapid, quantitative detection of CHK2 phosphorylated at threonine 68, as a readout of the ATM/CHK2 signaling pathway upon a DNA damage response (DDR)

In response to DNA damage, such as Double Strand Breaks (DSBs), ATM-Chk2 pathway and replication checkpoint responses are activated that mediate G1/S checkpoints to arrest cell cycle progression and allow extra time for DNA repair.

The activation of ATM results in the phosphorylation of the checkpoint kinase Chk2 at threonine 68. Chk2 is a stable protein expressed throughout the cell cycle, and it appears to be largely inactive in the absence of DNA damage. CHK2 activation involves its dimerization and autophosphorylation, and induces the activation of the downstream signal effectors such as the tumor suppressor protein p53 and CdC25C, and also controls cdk2/CyclinA activity.