Revvity HTRF Human & Mouse Total Cyclin D1 Detection Kit, 500 Assay Points

The Total Cyclin D1 cell-based assay conveniently and accurately detects the levels of total Cyclin D1 within cells. This kit is compatible with the buffers from the phospho-Cyclin D1 (Thr286) kit, SO the same lysate can be used for analyses of both the phosphorylated and the total protein populations.

Cyclin D family members (including Cyclin D1, Cyclin D2, and Cyclin D3) are important regulators of cell cycle progression. They are integral mediators of growth factor-dependent G1 to S phase progression by playing the role of allosteric regulators of CDK4/6.

Cyclin D1 is upregulated in presence of extracellular mitogenic stimuli, and is then rapidly downregulated by proteasomal degradation following its phosphorylation on Thr286 by the kinase GSK-3β.

Cyclin D1 is an oncogene which is overexpressed in many human cancers. Abnormal increased levels of Cyclin D1 result in dysregulated CDK4/6 activity, and lead to tumorigenesis. Cyclin D1 overexpression may be caused by gene amplification or chromosomal rearrangements, or be a result of impaired degradation of the protein.