Revvity HTRF Human & mouse TGFβ1 Detection Kit, 10,000 Assay Points

TGF beta 1 (Transforming Growth Factor Beta-1) is considered one of major cytokines involved in regulation of extracellular matrix (ECM) synsis and degradation, as well as a major profibrotic factor. TGF beta 1 pre-proprotein is processed to generate a latency-associated peptide (LAP) and a mature TGFß1 peptide, which remain associated through strong non-covalent interactions. An activation step (MMP2 cleavage or acid activation) is required to release active form of TGF beta 1 from TGFß1-LAP complex.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.