Revvity HTRF dsRNA (IVT) Kit,500 Assay Points

In Vitro Transcription or IVT is molecular biology technique used to synthesize RNA sequence from a DNA template. During that process, a DNA template is incubated with RNA polymerase and ribonucleotides, resulting in RNA copies of the template.

While this process aims at producing single-stranded RNA (ssRNA) sequences, it is also prone to generating double-stranded RNA (dsRNA) contaminants. These can arise for different reasons (polymerase pausing, secondary structure formation, template contamination, etc) and are undesirable in the final product.

The end-goal therapeutic applications of IVT are mostly related to gene therapy and mRNA vaccine development or production, and in many cases IVT applications are parts of production processes for patient-intended products. One of the main concerns when running such processes is the QA/QC aspect of it as well as guaranteeing patient safety with the end results. For that reason, dsRNA is a contaminant to monitor with great attention and robust tools, as it is usually a strongly immunogenic molecule that can be picked up by multiple nucleic acid receptors of our innate immunity and trigger violent immune and inflammatory responses in patients. Rapid, convenient, and robust quantification of dsRNA is therefore required at multiple purification steps of the IVT process to ensure maximum elimination of that contaminant.

This HTRF dsRNA (IVT) Kit is intended for the robust detection of dsRNA in such IVT samples. It was developed with IVT applications specifically in mind offers a ready-to-use homogeneous alternative to traditional ELISA and DOT-blot approaches.