Revvity HTRF Human & Mouse Phospho-CDK1 (Thr15) Detection Kit, 500 Assay Points

This HTRF cell-based assay conveniently and accurately detects phosphorylated CDK1 at Tyr15.

CDK1 (Cyclin-Dependent Kinase 1) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells (also including CDK1, CDK4, and CDK6). CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. CDK1 promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins (Cyclin A, Cyclin B). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of CDK1, can be carried out by Wee1 and Myt1 protein kinases. The cdc25 phosphatase may be responsible for the removal of phosphates at Thr14 and Tyr15 and subsequent activation of CDK1.

CDK1 inhibitory phosphorylation at Tyr15 is essential for maintaining genome integrity and preventing DNA damage during the S phase. The Wee1/Cdc25A axis is therefore an attractive target for cancer therapy and may represent a unique approach to sensitize cancer cells with hyperactive CDK1.