Revvity HTRF GAPDH Housekeeping Cellular Kit, 500 Assay Points

This assay is designed to measure the endogenous expression level of GAPDH in cells and tissues, in order to normalize data obtained on the phospho- and/or total protein(s) of interest. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a key glycolytic enzyme which is constitutively and stably expressed in all cells and tissues. Its amino acid sequence exhibits a high degree of homology between mammalian species. These characteristics make it one of the most commonly used housekeeping proteins. Its concentration is proportional to cell numbers and total protein concentrations, and it is therefore used as an internal control for data normalization, to correct for signal changes caused by experimental variability (e.g. number of cells remaining in the culture plate after treatment, or lysis efficacy). The low sample volume of 4 µL and its compatibility with all HTRF phospho-total protein lysis buffers enable a multi-parametric analysis from the same lysate, with the detection of GAPDH in parallel with the phospho- and/or total protein(s) of interest.